diff --git a/Metagenomics/Low_Biomass/Illumina/GL-DPPD-7117.md b/Metagenomics/Low_Biomass/Illumina/GL-DPPD-7117.md
index 0b2f6b94..2731ed88 100644
--- a/Metagenomics/Low_Biomass/Illumina/GL-DPPD-7117.md
+++ b/Metagenomics/Low_Biomass/Illumina/GL-DPPD-7117.md
@@ -285,11 +285,11 @@ kraken2 --db kraken2_human_db \
sample1_R1_raw.fastq.gz sample1_R2_raw.fastq.gz
# rename and gzip output files
-mv sample1_R_1.fastq sample1_R1_HRrm_GLlbsMetag.fastq && \
-gzip sample1_R1_HRrm_GLlbsMetag.fastq
+mv sample1_R_1.fastq sample1_GLlbsMetag_R1_HRrm.fastq && \
+gzip sample1_GLlbsMetag_R1_HRrm.fastq
-mv sample1_R_2.fastq sample1_R2_HRrm_GLlbsMetag.fastq && \
-gzip sample1_R2_HRrm_GLlbsMetag.fastq
+mv sample1_R_2.fastq sample1_GLlbsMetag_R2_HRrm.fastq && \
+gzip sample1_GLlbsMetag_R2_HRrm.fastq
```
**Parameter Definitions:**
@@ -312,7 +312,7 @@ gzip sample1_R2_HRrm_GLlbsMetag.fastq
- sample1-kraken2-output.txt (kraken2 read-based output file (one line per read))
- sample1-kraken2-report.tsv (kraken2 report output file (one line per taxa, with number of reads assigned to it))
-- **sample1_raw_HRrm_GLlbsMetag.fastq.gz** (raw sample reads with human reads removed, gzipped fasta file)
+- **sample1_GLlbsMetag_[R1|R2]_HRrm.fastq.gz** (raw sample reads with human reads removed, gzipped fasta file)
#### 2c. Compile Human Read Removal QC
@@ -377,7 +377,7 @@ fastp --in1 sample1_R1_raw.fastq.gz --out1 temp_sample1_R1_filtered.fastq.gz \
**Input Data:**
-- *raw_HRrm_GLlbsMetag.fastq.gz (raw sample reads with human reads removed, from [Step 2b](#2b-remove-human-reads))
+- *_GLlbsMetag_HRrm.fastq.gz (raw sample reads with human reads removed, from [Step 2b](#2b-remove-human-reads))
**Output Data:**
@@ -386,8 +386,8 @@ fastp --in1 sample1_R1_raw.fastq.gz --out1 temp_sample1_R1_filtered.fastq.gz \
#### 3b. Trim polyG
```bash
-fastp --in1 temp_sample1_R1_filtered.fastq.gz --out1 sample1_R1_filtered_GLlbsMetag.fastq.gz \
- --in2 temp_sample1_R2_filtered.fastq.gz --out2 sample1_R2_filtered_GLlbsMetag.fastq.gz \
+fastp --in1 temp_sample1_R1_filtered.fastq.gz --out1 sample1_GLlbsMetag_R1_filtered.fastq.gz \
+ --in2 temp_sample1_R2_filtered.fastq.gz --out2 sample1_GLlbsMetag_R2_filtered.fastq.gz \
--qualified_quality_phred 20 \
--length_required 50 \
--thread 2 \
@@ -418,7 +418,7 @@ fastp --in1 temp_sample1_R1_filtered.fastq.gz --out1 sample1_R1_filtered_GLlbsMe
**Output Data:**
-- **\*filtered_GLlbsMetag.fastq.gz** (quality filtered and adapter trimmed, human removed reads)
+- **\*_(filtered.fastq.gz** (quality filtered and adapter trimmed, human removed reads)
#### 3c. Filtered Data QC
@@ -429,11 +429,11 @@ fastqc -o filtered_fastqc_output *filtered.fastq.gz
**Parameter Definitions:**
- `-o` – the output directory to store results
-- `*filtered_GLlbsMetag.fastq.gz` – the input reads are specified as a positional argument, and can be given all at once with wildcards like this, or as individual arguments with spaces in between them
+- `*_GLlbsMetag_R[12]_filtered.fastq.gz` – the input reads are specified as a positional argument, and can be given all at once with wildcards like this, or as individual arguments with spaces in between them
**Input data:**
-- *filtered_GLlbsMetag.fastq.gz (trimmed and filtered reads, from [Step 3b](#3b-trim-polyg))
+- *filtered.fastq.gz (trimmed and filtered reads, from [Step 3b](#3b-trim-polyg))
**Output data:**
@@ -479,8 +479,8 @@ multiqc --zip-data-dir \
#### 4a. Assemble Contaminants
```bash
-cat /path/to/contaminant_fastq/*_R1_filtered_GLlbsMetag.fastq.gz > merged_R1.fastq.gz
-cat /path/to/contaminant_fastq/*_R2_filtered_GLlbsMetag.fastq.gz > merged_R2.fastq.gz
+cat /path/to/contaminant_fastq/*_GLlbsMetag_R1_filtered.fastq.gz > merged_R1.fastq.gz
+cat /path/to/contaminant_fastq/*_GLlbsMetag_R2_filtered.fastq.gz > merged_R2.fastq.gz
spades.py --meta \
--threads 8 \
@@ -503,7 +503,7 @@ mv spades.log blank-assembly.log
**Input Data**
-- *_R[12]_filtered_GLlbsMetag.fastq.gz (one or more paired-end, trimmed and filtered, HRrm reads from blank (negative control) samples, output from [Step 3b](#3b-trim-polyg))
+- *_GLlbsMetag_R[12]_filtered.fastq.gz (one or more paired-end, trimmed and filtered, HRrm reads from blank (negative control) samples, output from [Step 3b](#3b-trim-polyg))
**Output Data**
@@ -522,14 +522,14 @@ bowtie2-build /path/to/contaminant_assembly/blank-scaffolds.fasta /path/to/blank
bowtie2 -p NumberOfThreads \
-x /path/to/blank-index/blanks \
--very-sensitive-local \
- -1 sample1_R1_filtered_GLlbsMetag.fastq.gz \
- -2 sample2_R2_filtered_GLlbsMetag.fastq.gz \
+ -1 sample1_GLlbsMetag_R1_filtered.fastq.gz \
+ -2 sample2_GLlbsMetag_R2_filtered.fastq.gz \
--un-conc-gz sample1_decontam.fastq.gz
> sample1.sam 2> sample1-mapping-info.txt
# rename blank removed fastq files
-mv sample1_decontam.fastq.1.gz sample1_R1_decontam_GLlbsMetag.fastq.gz
-mv sample1_decontam.fastq.2.gz sample1_R2_decontam_GLlbsMetag.fastq.gz
+mv sample1_decontam.fastq.1.gz sample1_GLlbsMetag_R1_decontam.fastq.gz
+mv sample1_decontam.fastq.2.gz sample1_GLlbsMetag_R2_decontam.fastq.gz
# remove intermediate file
rm -rf sample1.sam
@@ -554,11 +554,11 @@ rm -rf sample1.sam
**Input Data**
- /path/to/contaminant_assembly/blank-scaffolds.fasta (contaminant assembly, output from [Step 4a](#4a-assemble-contaminants))
-- sample1_R[12]_filtered_GLlbsMetag.fastq.gz (filtered and trimmed reads, output from [Step 3b](#3b-trim-polyg))
+- sample1_GLlbsMetag_R[12]_filtered.fastq.gz (filtered and trimmed reads, output from [Step 3b](#3b-trim-polyg))
**Output Data**
-- sample1_R[12]_decontam_GLlbsMetag.fastq.gz (decontaminated reads)
+- sample1_GLlbsMetag_R[12]_decontam.fastq.gz (decontaminated reads)
- sample-mapping-info.txt (bowtie2 mapping log file)
@@ -566,17 +566,17 @@ rm -rf sample1.sam
#### 4c. Contaminant Removal QC
```bash
-fastqc -o decontam_fastqc_output *decontam_GLlbsMetag.fastq.gz
+fastqc -o decontam_fastqc_output *decontam.fastq.gz
```
**Parameter Definitions:**
- `-o` – the output directory to store results
-- `*decontam_GLlbsMetag.fastq.gz` – the input reads are specified as a positional argument, and can be given all at once with wildcards like this, or as individual arguments with spaces in between them
+- `*decontam.fastq.gz` – the input reads are specified as a positional argument, and can be given all at once with wildcards like this, or as individual arguments with spaces in between them
**Input data:**
-- *decontam_GLlbsMetag.fastq.gz (decontaminated reads)
+- *decontam.fastq.gz (decontaminated reads)
**Output data:**
@@ -673,15 +673,15 @@ kraken2 --db kraken2_${hostname}_db \
--use-names \
--output sample-kraken2-output.txt \
--report sample-kraken2-report.tsv \
- --unclassified-out sample1_R#.fastq \
- sample1_R1_decontam.fastq.gz sample1_R2_decontam.fastq.gz
+ --unclassified-out sample1_GLlbsMetag_R#.fastq \
+ sample1_GLlbsMetag_R1_decontam.fastq.gz sample1_GLlbsMetag_R2_decontam.fastq.gz
# rename and gzip output files
-mv sample1_R_1.fastq sample1_R1_HostRm_GLlbsMetag.fastq && \
-gzip sample1_R1_HostRm_GLlbsMetag.fastq
+mv sample1_R_1.fastq sample1_GLlbsMetag_R1_HostRm.fastq && \
+gzip sample1_GLlbsMetag_R1_HostRm.fastq
-mv sample1_R_2.fastq sample1_R2_HostRm_GLlbsMetag.fastq && \
-gzip sample1_R2_HostRm_GLlbsMetag.fastq
+mv sample1_R_2.fastq sample1_GLlbsMetag_R2_HostRm.fastq && \
+gzip sample1_GLlbsMetag_R2_HostRm.fastq
```
**Parameter Definitions:**
@@ -693,18 +693,18 @@ gzip sample1_R2_HostRm_GLlbsMetag.fastq
- `--output` - Specifies the name of the kraken2 read-based output file (one line per read).
- `--report` - Specifies the name of the kraken2 report output file (one line per taxa, with number of reads assigned to it).
- `--unclassified-out` - Specifies the name of the output file containing reads that were not classified, i.e non-host reads.
-- `sample1_R1_decontam_GLlbsMetag.fastq.gz sample1_R2_decontam_GLlbsMetag.fastq.gz` - Positional argument specifying the input read files.
+- `sample1_GLlbsMetag_R1_decontam.fastq.gz sample1_GLlbsMetag_R2_decontam.fastq.gz` - Positional argument specifying the input read files.
**Input Data:**
- kraken2_host_db/ (kraken2 host database directory, output from [Step 5a](#5a-build-kraken2-host-database))
-- sample_*decontam_GLlbsMetag.fastq.gz (filtered and trimmed sample reads with contaminants removed, output from [Step 4b](#4b-build-contaminant-index-and-map-reads))
+- sample_GLlbsMetag_R[12]_decontam.fastq.gz (filtered and trimmed sample reads with contaminants removed, output from [Step 4b](#4b-build-contaminant-index-and-map-reads))
**Output Data:**
- sample-kraken2-output.txt (kraken2 read-based output file (one line per read))
- sample-kraken2-report.tsv (kraken2 report output file (one line per taxa, with number of reads assigned to it))
-- **sample_HostRm_GLlbsMetag.fastq.gz** (filtered and trimmed sample reads with contaminants, human, and host reads removed, gzipped fasta file)
+- **sample_GLlbsMetag_HostRm.fastq.gz** (filtered and trimmed sample reads with contaminants, human, and host reads removed, gzipped fasta file)
#### 5c. Compile Host Read Removal QC
@@ -1620,8 +1620,8 @@ kaiju -f kaiju-db/nr_euk/kaiju_db_nr_euk.fmi \
-t kaiju-db/nodes.dmp \
-z NumberOfThreads \
-E 1e-05 \
- -i /path/to/sample1_R1_decontam_GLlbsMetag.fastq.gz \
- -j /path/to/sample1_R2_decontam_GLlbsMetag.fastq.gz \
+ -i /path/to/sample1_GLlbsMetag_R1_decontam.fastq.gz \
+ -j /path/to/sample1_GLlbsMetag_R2_decontam.fastq.gz \
-o sample_kaiju.out
```
@@ -2034,7 +2034,7 @@ kraken2 --db kraken2-db/ \
--use-names \
--output sample-kraken2-output.txt \
--report sample-kraken2-report.tsv \
- /path/to/sample1_R1_decontam_GLlbsMetag.fastq.gz /path/to/sample1_R2_decontam_GLlbsMetag.fastq.gz
+ /path/to/sample1_GLlbsMetag_R1_decontam.fastq.gz /path/to/sample1_GLlbsMetag_R2_decontam.fastq.gz
```
**Parameter Definitions:**
@@ -2045,8 +2045,8 @@ kraken2 --db kraken2-db/ \
- `--use-names` - Specifies to add taxa names in addition to taxids.
- `--output` - Specifies the name of the kraken2 read-based output file.
- `--report` - Specifies the name of the kraken2 report output file.
-- `sample1_R1_decontam_GLlbsMetag.fastq.gz` - Positional argument specifying the forward read input file.
-- `sample1_R2_decontam_GLlbsMetag.fastq.gz` - Positional argument specifying the reverse read input file.
+- `sample1_GLlbsMetag_R1_decontam.fastq.gz` - Positional argument specifying the forward read input file.
+- `sample1_GLlbsMetag_R2_decontam.fastq.gz` - Positional argument specifying the reverse read input file.
**Input Data:**
@@ -2401,7 +2401,7 @@ metaphlan --install
```bash
# forward and reverse reads need to be provided combined if paired-end (if not paired-end, single-end reads are provided to the --input argument next)
-cat sample1_R1_decontam_GLlbsMetag.fastq.gz sample1_R2_decontam_GLlbsMetag.fastq.gz > sample1-combined.fastq.gz
+cat sample1_GLlbsMetag_R1_decontam.fastq.gz sample1_GLlbsMetag_R2_decontam.fastq.gz > sample1-combined.fastq.gz
humann --input sample1-combined.fastq.gz \
--output sample1-humann3-out-dir \
@@ -2431,7 +2431,7 @@ mv sample1-humann3-out-dir/sample1_humann_temp/sample1_metaphlan_bugs_list.tsv \
**Input Data:**
- `/path/to/humann3-db/` (HUMAnN databases installed in [Step 9a](#9a-download-and-install-humann-databases))
-- *_R[12]_decontam_GLlbsMetag.fastq.gz or *_R[12]_HostRm_GLlbsMetag.fastq.gz (filtered and trimmed sample reads with both
+- *_GLlbsMetag_R[12]_decontam.fastq.gz or *_GLlbsMetag_R[12]_HostRm.fastq.gz (filtered and trimmed sample reads with both
contaminants and human reads (and, optionally, host reads) removed, gzipped fasta file,
output from [Step 4b](#4b-build-contaminant-index-and-map-reads) or [Step 5b](#5b-remove-host-reads))
@@ -2858,7 +2858,7 @@ htmlwidgets::saveWidget(ggplotly(p), glue("Metaphlan_decontam_species_barplot_GL
### 10. Sample Assembly
```
-megahit -1 sample1_R1_decontam_GLlbsMetag.fastq.gz -2 sample1_R2_decontam_GLlbsMetag.fastq.gz \
+megahit -1 sample1_GLlbsMetag_R1_decontam.fastq.gz -2 sample1_GLlbsMetag_R2_decontam.fastq.gz \
-o sample1-assembly -t NumberOfThreads --min-contig-length 500 > sample1-assembly.log 2>&1
```
@@ -2873,7 +2873,7 @@ megahit -1 sample1_R1_decontam_GLlbsMetag.fastq.gz -2 sample1_R2_decontam_GLlbsM
**Input data:**
-- *_R[12]_decontam_GLlbsMetag.fastq.gz or *_R[12]_HostRm_GLlbsMetag.fastq.gz (filtered and trimmed sample reads with both
+- *_GLlbsMetag_R[12]_decontam.fastq.gz or *_GLlbsMetag_R[12]_HostRm.fastq.gz (filtered and trimmed sample reads with both
contaminants and human reads (and, optionally, host reads) removed, gzipped fasta file,
output from [Step 4b](#4b-build-contaminant-index-and-map-reads) or [Step 5b](#5b-remove-host-reads))
@@ -3260,8 +3260,8 @@ bowtie2-build sample1_assembly_GLlbsMetag.fasta sample1-index
```bash
bowtie2 --mm --quiet --threads ${task.cpus} \
-x sample1-index \
- -1 sample1_R1_decontam_GLlbsMetag.fastq.gz \
- -2 sample1_R2_decontam_GLlbsMetag.fastq.gz \
+ -1 sample1_GLlbsMetag_R1_decontam.fastq.gz \
+ -2 sample1_GLlbsMetag_R2_decontam.fastq.gz \
--no-unal > sample1.sam 2> sample1-mapping-info_GLlbsMetag.txt
```
@@ -3280,7 +3280,7 @@ bowtie2 --mm --quiet --threads ${task.cpus} \
**Input Data**
- sample1-index (bowti2 index files, output from [Step 15a](#15a-build-reference-index))
-- *_R[12]_decontam_GLlbsMetag.fastq.gz or *_R[12]_HostRm_GLlbsMetag.fastq.gz (filtered and trimmed sample reads with both
+- *_GLlbsMetag_R[12]_decontam.fastq.gz or *_GLlbsMetag_R[12]_HostRm.fastq.gz (filtered and trimmed sample reads with both
contaminants and human reads (and, optionally, host reads) removed, gzipped fasta file,
output from [Step 4b](#4b-build-contaminant-index-and-map-reads) or [Step 5b](#5b-remove-host-reads))
diff --git a/Metagenomics/Low_Biomass/Nanopore/GL-DPPD-7116.md b/Metagenomics/Low_Biomass/Nanopore/GL-DPPD-7116.md
index 2a2ae9e0..a8bb2178 100644
--- a/Metagenomics/Low_Biomass/Nanopore/GL-DPPD-7116.md
+++ b/Metagenomics/Low_Biomass/Nanopore/GL-DPPD-7116.md
@@ -376,7 +376,7 @@ filtlong --min_length 200 --min_mean_q 8 /path/to/raw_data/sample.fastq.gz > sam
```bash
NanoPlot --only-report \
- --prefix sample_filtered_ \
+ --prefix sample_filtered_GLlblMetag_ \
--outdir /path/to/filtered_nanoplot_output \
--threads NumberOfThreads \
--fastq \
@@ -398,7 +398,7 @@ NanoPlot --only-report \
**Output Data:**
-- **/path/to/filtered_nanoplot_output/sample_filtered_NanoPlot-report_GLlblMetag.html** (NanoPlot html summary)
+- **/path/to/filtered_nanoplot_output/sample_filtered_GLlblMetag_NanoPlot-report.html** (NanoPlot html summary)
- /path/to/filtered_nanoplot_output/sample_filtered_NanoPlot_\_\