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…or grouped fasta file generation
…combination analysis
Signed-off-by: Praneeth <pgangavarapu@scripps.edu>
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This pull request introduces several significant updates to the pipeline, including the addition of new modules and workflows, improvements to existing processes, and code standardization. Below is a summary of the most important changes grouped by theme:
New Modules and Workflows
VIREMAmodule: Includes a newVIREMAprocess for viral recombination analysis, along with its correspondingenvironment.ymlfile. This process outputs BED, BAM, and SAM files, as well as coverage statistics. (modules/local/virema/main.nf,modules/local/virema/environment.yml) [1] [2]GROUP_FASTAmodule: Introduced a new process to group FASTA files based on metadata. (modules/local/group_fasta/main.nf)DVGworkflow: Implements a workflow for detecting defective viral genomes, integrating theVIREMAprocess and other modules. (subworkflows/local/dvg/main.nf)COMBINE_SAMPLE_CONSENSUSworkflow: Combines sample consensus sequences into grouped FASTA files using theGROUP_FASTAmodule. (subworkflows/local/utils_nfcore_flusra_pipeline/main.nf)Enhancements to Existing Workflows
PROCESS_SRAworkflow: Integrated theDVGandCOMBINE_SAMPLE_CONSENSUSworkflows, replacing direct consensus handling with a more modular approach. (subworkflows/local/process_sra/main.nf) [1] [2] [3]MILK_FREYJAworkflow: Fixed a trailing comma in parameter handling. (subworkflows/local/milk_freyja/main.nf)Code Standardization
$variablewith${variable}across multiple modules for improved readability and consistency. (modules/nf-core/bwa/mem/main.nf,modules/nf-core/fastp/main.nf,modules/nf-core/freyja/demix/main.nf,modules/nf-core/ivar/variants/main.nf, etc.) [1] [2] [3] [4] [5]modules/nf-core/fastp/main.nf) [1] [2]These changes collectively enhance the pipeline's modularity, maintainability, and functionality.